Chemical Biology and Molecular Medicine at IISc, Bangalore
The Chemical Biology Group consists of research groups at the Indian Institute of Science, Bangalore, that share common interests in research areas at the interface of chemistry and biology. The group is interested in using small molecules and nanomaterials produced through synthetic chemistry to regulate/manipulate biological systems and in applying chemical techniques to study biological pathways. The approach involving chemistry applied to biology will help in understanding major disorders such as cardiovascular, endocrine, metabolic and neurological diseases and in designing small molecules and nanomaterials that can modulate the biological pathways under these conditions. The main objective of this interdisciplinary groups is to develop novel therapeutic strategies for the treatment of above diseases.
The expertise available within the ChemBiol group include, multistep organic and inorganic synthesis, chromatography and various spectroscopic and mass spectrometric techniques, bioinorganic chemistry to understand the role of metalloproteins including membrane-bound proteins, development of novel probes for the detection of biomolecules, various drug delivery methods, study of physiological and pathological angiogenesis and translational regulation of gene expression, cellular models for small-molecule-mediated redox regulation, primary cell cultures, transgenic and surgical mouse models to test small molecules and proteins for their ability to protect against cardiovascular diseases, studies of proteins involved in cardiac hypertrophy, fibrosis, mitochondrial dysfunction and muscle degeneration.
The facilities available for the multi-disciplinary work include: fume hoods and Schlenk lines to carry out air and moisture sensitive reactions, rotary evaporators, solvent distillation setup, refrigerators, hot-air oven, vacuum pumps, water circulation systems and flash chromatography system for purification of organic compounds. The instrumentation facilities in our laboratories include HPLC and UPLC systems, LC-MS facility, peptide synthesizer, water purification system. A computer cluster for theoretical calculations is also available. In addition to these facilities, our groups have access to 400/500 MHz NMR Spectrometers, various microscopes (AFM, SEM, TEM), single crystal and powder X-ray diffractometers, fluorescence, UV-Vis, FT-IR and FT-Raman spectrophotometers, MALDI-TOF, elemental analyzer etc. For the biology work, instruments such as gel electrophoresis, plate readers, blotting, thermal cycler, Gel-doc and chemiluminescence detection system, refrigerated centrifuges, protein purification system, deep freezers, lyophilizers, autoclave, orbital shaker, fluorescence microscope, CO2 incubators etc are available. We also have cell culture facilities to handle bacterial and mammalian cells.